Sorachim
Reference: COO-311

Cholesterol oxidase from Streptomyces sp.

Cholesterol oxidase from Streptomyces sp. Cholesterol + O2 ----> Choleste-4-en-3-one + H2O2 This enzyme is used for enzymatic determination of cholesterol when coupled with cholesterol esterase in clinical analysis.
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DESCRIPTION
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  • Product name: Cholesterol: oxygen oxidoreductase
  • Appearance: Yellowish lyophilized powder
  • Activity: Grade Ⅲ 15 U/mg or more(containing approx. 40% of
    stabilizers)
  • Contaminants: Catalase: < 1.0 % ,
  • Cholesterol esterase: < 0.01 %
  • Stability: Stable at - 20 °C for at least six months
  • Stabilizers: Bovine serum albumin, sugars
  • Molecular weight: approx. 34 000(by gel-filtration)
  • Isoelectric point: 5.1 ± 0.1 and 5.4 ± 0.1
  • Michaelis constants: 2.1 x 10-5 M (Cholesterol)
  • Inhibitors: Ionic detergents, Ag+, Hg2+
  • Optimum pH: 6.5 - 7.0
  • Optimum temperature: 45-50 °C
  • pH Stability: pH 5.0 - 10.0 (25°C, 20hr)
  • Thermal stability: below 45 °C (pH 7.0, 15 min)
  • EC # 1.1.3.6

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