Sorachim
Reference: GLD-351

Glucose-dehydrogenase (FAD-dependent)

Glucose-dehydrogenase (FAD-dependent) from Microorganism D-Glucose:(flavine adenine dinucleotide)-dehydrogenase EC # 1.1.99.10
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DESCRIPTION
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  • Name: D-Glucose:(flavine adenine dinucleotide)-dehydrogenase
  • EC 1.1.99.10
  • Appearance: Yellowish amorphous powder lyophilized
  • Activity: Grade III, 500U/mg-solid or more
  • Contaminants: Glucose dehydrogenase < 2.0×10-2 % (NAD-dependent)
  • Hexokinase < 2.0×10-2 %, Glucose oxidase < 2.0x10-2 %
  • Glucoamylase < 1.0x10-2 %
  • Stability: Stable at - 20°C for at least 12 months
  • Molecular weight: approx. 100,000 (by gel filtration)
  • Michaelis constant: 4.8 mM (D-Glucose)
  • Inhibitors: Cu2+, Pb2+, Ag+
  • Optimum pH 7.0
  • Optimum temperature 47°C
  • pH Stability 3.5 - 8.5 (25°C, 16hr)
  • Temperature stability below 50°C (pH 7.5, 30min)

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