Reference: GYK-301

Glycerol kinase

Glycerol + ATP ----> Glycerol-3-phosphate + ADPMg2+ Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2+
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DESCRIPTION
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This enzyme is used for enzymatic determination of glycerol and triglyceride
when coupled with glycerol-3-phosphate dehydrogenase G-3-P DH (G3D-301), glycerol-3-phosphate oxidase G-3-P oxidase (G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.

  • Product name ATP: Glycerol 3-phosphotransferase
  • Appearance White amorphous powder lyophilized
  • Activity Grade III 20 U/mg-solid or more (containing approx. 50% stabilizers)
  • Contaminants NADH oxidase ≤ 1.0×10-1 %
  • Catalase ≤ 1.0×10-2 %
  • Phosphatase (pH 6) ≤ 1.0×10-3 %
  • Stability Stable at - 20℃ for at least one year
  • Molecular weight approx. 128,000 (by gel filtration)
  • Isoelectric point 4.2
  • Michaelis constants 4.4×10-5 M (Glycerol), 4.3×10-4M (ATP)
  • Inhibitors p-Chloromercuribenzoate, heavy metal ions (Pb2+, Fe2+, Hg2+, Ag+)
  • Optimum pH 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)
  • Optimum temperature 50 °C
  • pH Stability pH 5.5 - 10.0 (25 °C, 20hr)
  • Thermal stability below 40 °C (pH 7.5, 15min)
  • Substrate specificity This enzyme catalyzes the stereospecific transfer of the terminal phosphoryl
  • moiety of ATP to one of the primary hydroxyl group of glycerol, forming snglycerol-
  • 3-P. The enzyme has the highest specificity for glycerol, and also
  • phosphorylates dihydroxyacetone and glyceraldehyde. Mg2+ is essentially
  • required for the reaction.
  • EC # 2.7.1.30

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