Reference: LAX-18C

Lactate oxidase

Lactate oxidase from Microorganism L-Lactate + O2 ----> Pyruvate + H2O2 L-Lactate + O2 <---- Pyruvate + H2O2 This enzyme is used for enzymatic determination of L-Lactate when coupled with the related enzymes.
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DESCRIPTION
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  • Product name: L-Lactate: oxygen oxidoreductase
  • Appearance: Yellowish lyophilized powder
  • Activity: > 20 U/mg lyophilizate
  • Specific activity: > 55 U/mg protein
  • Contaminants: Pyruvate oxidase: < 0.001 %
  • Uricase: < 0.001 %
  • Glucose oxidase: < 0.001 %
  • Protein (BCA): 0.3 - 0.7 mg/mg lyophilizate
  • Stability: Stable at - 20 °C for at least one year
  • Michaelis constant: L-lactate 5 x 10-4 mol/l
  • Vmax: L-lactate 0.2 mol/l
  • Optimum pH: 6.5 - 7.5
  • pH Stability: 6.0 - 9.0
  • Thermal stability: below 65 °C
  • EC # 1.13.12.4

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