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Phospholipase D
Reference: PHO-12C

Phospholipase D

Phospholipase D from Streptomyces chromofuscus Phosphatidylcholine + H2O ---> Phosphatidic acid + Choline This enzyme is used for enzymatic determination of phospholipids in clinical analysis. If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
Available quantities:
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
DESCRIPTION
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  • Name: Phosphatidylcholine phosphatidohydrolase
  • Appearance: Brown powder lyophilized
  • Activity: 40U/mg-solid or more
  • Specific activity: 150U/mg protein or more
  • Stability: Stable at -20°C for at least 12 months
  • Molecular weight: approx. 57 000 by SDS electrophoresis
  • Isoelectric point: 5.1
  • Michaelis constant: 1.4×10-3 M (phosphatidylcholine)
  • Inhibitors: EDTA
  • Optimum: pH 7.0—8.5
  • pH stability: 6.0—10.0
  • Thermal Stability: Stable at below 60°C (pH 8.0, 10min)
  • Activators: Ca, Triton X-100, DOC, Adekatol S0-120
  • Contaminating: enzymes Catalase : < 0.6 % - Glucose oxidase : < 0.02 %
  • EC # 3.1.4.4
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