Sorachim
Reference: COO-122S

Cholesterol oxidase from Microorganism

Cholesterol oxidase from Microorganism Cholesterol + O2 ----> Choleste-4-en-3-one + H2O2 This enzyme is used for enzymatic determination cholesterol in serum when coupled with cholesterol esterase in clinical analysis.
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DESCRIPTION
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  • Product name: Cholesterol: oxygen oxidoreductase
  • Appearance: Yellow to brownish lyophilized powder
  • Activity: 25 U/mg or more
  • Contaminants: Catalase: < 1 %
  • Cholesterol esterase: < 0.1 %
  • Glucose oxidase: < 0.1 %
  • Stability: Stable at - 20 °C for at least one year
  • Stabilizers: Bovine serum albumin, amino acids
  • Molecular weight: Approx. 56,000 (by gel-filtration)
  • Michaelis constants: 2 x 10-6M (Cholesterol)
  • Inhibitors: Ionic detergents, Hg2+
  • Optimum pH: 6.5 - 8.0
  • Optimum temperature: 55 °C
  • pH Stability: pH 4.5 - 10.0 (25°C, 20hr)
  • Thermal stability: Below 60°C (pH 7.0, 15 min)
  • EC # 1.1.3.6

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