Glycerol kinase

Reference: GYK-311

Glycerol kinase

Glycerol kinase from Microorganism Glycerol + ATP ----> Glycerol-3-phosphate + ADPMg2⁺ Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2⁺

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DESCRIPTION

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Product name: ATP: Glycerol 3-phosphotransferase
Appearance: White amorphous powder lyophilized
Activity: Grade Ⅲ 30 U/mg-solid or more
Contaminants: Catalase : ≤ 1.0x10^-1 %, NADH oxidase : ≤ 1.0x10^-3 %
Adenosine triphosphate : ≤ 1.0x10^-3 %
Stability: Stable at -20°C for at least 12 months
Molecular weight: approx. 220,000 (by gel filtration)
Structure: 4 subunits of approx 58,000
Isoelectric poin:t 4.3
Michaelis constants: 9.4×10^-5 M (Glycerol), 1.3×10^-5 M (ATP), 2.1x10^-3 M (dihydroxiacetone)
Inhibitors: p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺
Optimum pH: 10.0
Optimum temperature: 70℃
pH Stability: pH 5.5 - 10.0 (25°C, 20hr)
Thermal stability: below 65℃ (pH 7.5, 30min)
EC # 2.7.1.30

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