Purine-nucleoside phosphorylase

Reference: PNP-311

Purine-nucleoside phosphorylase

Purine-nucleoside : orthophosphate ribosyltransferase from Microorganism EC 2.4.2.1

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DESCRIPTION

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Name: Purine-nucleoside: orthophosphate ribosyltransferase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 15 U/mg-solid or more
Contaminants: Catalase ≤ 20%
5'-Nucleosidase ≤ 1.0×10^-3 %
Adenosine deaminase ≤ 1.0×10^-3 %
ATPase ≤ 1.0×10^-2 %
Stabilizers: K-Gluconate, mannitol, EDTA
Stability: Stable at - 20℃ for at least 12 months
Molecular weight: Approx. 120,000
Isoelectric point: 4.1±0.1
Michaelis constants: 6.4×10^-5 M (Inosine), 3.2×10^-4 M (Pi)
Inhibitors: p-Chloromercuribenzoate, SDS, Hg²⁺, Ag⁺
Optimum pH: 7.5－8.0
Optimum temperature: 65℃
pH Stability: pH 6.0－9.0 (30℃, 16hr)
Thermal stability: Below 60℃ (pH 7.7, 30min)
EC # 2.4.2.1

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