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Glucose-dehydrogenase (NADP(P)-dependent)
Reference: GLD-311

Glucose-dehydrogenase (NADP(P)-dependent)

Glucose-dehydrogenase (NADP(P)-dependent) from Microorganism β-D-Glucose + NAD(P)+ ----> D-glucono-δ-Lactone + NAD(P)H + H+ This enzyme is used for enzymatic determination of D-Glucose
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DESCRIPTION
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  • Name: β-D-Glucose: NAD(P)+1-oxydoreductase
  • Appearance: White amorphous powder lyophilized
  • Activity: Grade III, 250U/mg-solid or more
  • Contaminants: NADH oxidase ≤ 1.0×10-3 %
    α-Glucosidase ≤ 1.0×10-3 %
    Glucose-6-phosphate dehydrogenase ≤ 1.0×10-3 %
  • Stability: Stable at - 20°C for at least one year
  • Molecular weight: approx. 101,000 (Gel filtration)
  • Isoelectric point: 4.5
  • Michaelis constants: NAD＋linked : 1.38×10-2 M (D-Glucose) 3.09×10-4 M (NAD+)
    NADP＋linked : 1.25×10-2M (D-Glucose) 4.07×10-5 M (NADP+)
  • Inhibitors: Ag+, Hg2+, Monoiodoacetate
  • Optimum pH: 9.0
  • Optimum temperature: 55°C
  • pH Stability: pH 6.0－7.5 (20°C, 16hr)
  • Thermal stability: 45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)
  • Substrate specificity: Specific for β-D-Glucose or 2-Deoxy-glucose (Either NAD+ or NADP+ serves as coenzyme.)
  • EC # 1.1.1.47
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