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Creatinase
Reference: CRH-221

Creatinase from Microorganism

Creatinase from Actinobacillus sp. Creatine + H20 Sarcosine + Urea This enzyme is used for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase (CNH-211, CNH-311) sarcosine dehydrogenase or sarcosine oxidase (SAO-341) in clinical analysis.
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DESCRIPTION
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  • Appearance: White amorphous powder lyophilized
  • Activity: Grade II 4.0U/mg-solid or more (containing approx. 50% of stabilizers)
  • Contaminant: NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0%
  • Stabilizers: Sugars, EDTA
  • Stability: Stable at－20℃ for at least 12 month
  • Molecular weight: approx. 100,000
  • Isoelectric point: 4.5±0.1
  • Michaelis constant: 4.5×10-2M (Creatine)
  • Inhibitors: Cu2+, Hg2+, Ag＋, SH reagent (NEM), PCMB
  • Optimum pH: 6.5－7.5
  • Optimum temperature: 40-50℃
  • pH Stability: pH 4.0－10.0 (25℃,20hr)
  • Thermal stability: below 50℃ (pH 7.5, 30min)
  • EC # 3.5.3.3
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