Reference: LPP-209

Alkaline phosphatase

Alkaline phosphatase from Calf intestine Orthophosphoric monoester + H2O ----> Alcohol + Orthophosphate This enzyme is used in molecular biology and in immuo-assays.
Available quantities:
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
DESCRIPTION
Download
  • Product name: Orthophosphoric monoester
  • phosphohydrolase (alkaline optimum)
  • Appearance: 50% glycerol solution
  • Activity: Grade II - 30,000 U/ml
  • 2,500 U/mg-protein or more
  • Contaminants: Adenosine deaminase ≤ 1.0×10-4 %
  • Phosphodiesterase ≤ 1.0×10-3 %
  • DNase*
  • RNase**
  • Stability Store at - 20 °C
  • Molecular weight approx. 100,000
  • Isoelectric point 5.7
  • Michaelis constant 1.7×10-3M (p-Nitrophenyl phosphate)
  • Inhibitors Cu2+, Ag+, Hg2+, EDTA
  • Optimum pH 10.0 - 10.3
  • Optimum temperature 40 °C
  • pH Stability pH 8.5 - 10.3 (25 °C, 20hr)
  • Thermal stability below 40 °C (pH 9.5, 30min)
  • * No degradation of the fragments is observed by agarose gel electrophoresis, after incubation of 1μg of λ-DNA
  • with 2 units of alkaline phosphatase for 16 hr at 37℃ in a 50μl reaction volume.
  • ** No degradation of the fragments is observed by polyacrylamide gel electrophoresis, after incubation of 2μg
  • of t-RNA with 2 units of alkaline phosphatase for 16 hr 37℃ in a 50μl reaction volume.
  • EC # 3.1.3.1

We will solve any doubts you may have

Powered by VOLCÀNIC INTERNET
envelopephone-handset