Reference: ASO-311

Ascorbate oxidase from Cucurbita sp.

Ascorbate oxidase from Cucurbita sp. L-Ascorbic acid + 1/2O₂ ----> Dehydroascorbic acid + H₂O This enzyme is useful for enzymatic determination of ascorbic acid and for eliminating the interferences of ascorbic acid in clinical analysis.

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DESCRIPTION

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Appearance : Light blue amorphous powder, lyophilized
Activity : GradeⅢ　200U/mg-solid or more
Contaminants : Catalase <1.0 Phosphatase <0.02%
Stabilizers : Bovine serum albumin, borate.
Stability : Store at －20℃
Molecular weight : Approx. 140,000
Michaelis constant : 2.7×10^－4 M (Ascorbate)
Inhibitors : cyanide, Na₂S, diethyldithiocarbamate (Na)
Optimum pH : 5.5 - 6.5
Optimum temperature : approx. 45℃
pH Stability : pH 6.0－10.0 (25℃, 20hr)
Thermal stability : below 45℃ (pH 7.0, 30min)
Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
EC # 1.10.3.3

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Ascorbate oxidase from Cucurbita sp.

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