Sorachim
Reference: ASO-311

Ascorbate oxidase from Cucurbita sp.

Ascorbate oxidase from Cucurbita sp. L-Ascorbic acid + 1/2O2 ----> Dehydroascorbic acid + H2O This enzyme is useful for enzymatic determination of ascorbic acid and for eliminating the interferences of ascorbic acid in clinical analysis.
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DESCRIPTION
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  • Appearance : Light blue amorphous powder, lyophilized
  • Activity : GradeⅢ 200U/mg-solid or more
  • Contaminants : Catalase <1.0 Phosphatase <0.02%
  • Stabilizers : Bovine serum albumin, borate.
  • Stability : Store at -20℃
  • Molecular weight : Approx. 140,000
  • Michaelis constant : 2.7×10-4 M (Ascorbate)
  • Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
  • Optimum pH : 5.5 - 6.5
  • Optimum temperature : approx. 45℃
  • pH Stability : pH 6.0-10.0 (25℃, 20hr)
  • Thermal stability : below 45℃ (pH 7.0, 30min)
  • Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
  • EC # 1.10.3.3

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