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Ascorbate oxidase
Reference: ASO-301

Ascorbate oxidase from Cucumis sp.

Ascorbate oxidase from Cucumis sp. L-Ascorbic acid + 1/2O2 ----> Dehydroascorbic acid + H2O This enzyme is used for enzymatic determination of ascorbic acid and for eliminating the interferences of ascorbic acid in clinical analysis.
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DESCRIPTION
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  • Appearance : Light blue amorphous powder, lyophilized
  • Activity : GradeⅢ　200U/mg-solid or more (containing approx. 70% of stabilizers)
  • Contaminants : Catalase ≤1.0×10－1 % Phosphatase ≤2.0×10－2 %
  • Stabilizers : Bovine serum albumin (BSA), borax, basic amino acids.
  • Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.)
  • Molecular weight : Approx. 140,000
  • Isoelectric point : between 6.0 and 7.8.8 2
  • Michaelis constant : 2.5×10－4 M (Ascorbate)
  • Structure : 8 copper atoms per mol of enzyme 1,2
  • Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
  • Optimum pH : 5.6
  • Optimum temperature : approx. 30℃
  • pH Stability : pH 7.0－10.0 (25℃, 17hr)
  • Thermal stability : below 40℃ (pH 8.0, 30min)
  • Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
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