Sorachim
Reference: COE-05C

Cholesterol esterase from Porcine pancreas

Cholesterol esterase from Porcine pancreas Cholesterol ester + H2O ----> Cholesterol + fatty acid Cholesterol ester + H2O <---- Cholesterol + fatty acid This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase (COO-311, COO-321) in clinical analysis.
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DESCRIPTION
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  • Product name: Steryl-ester Acylhydrolase
  • Appearance Light brown amorphous powder lyophilized
  • Activity Grade III 100U/mg-solid or more (containing approx. 40% of
    stabilizers)
  • Contaminant Catalase ≤1.0×10-2%
  • Stabilizers Mg2+ Na-cholate bovine serum albumin
  • Stability Stable at - 20℃ for at least one year
  • Molecular weight approx. 300,000
  • Isoelectric point 5.9 ± 0.1
  • Michaelis constants 5.4×10-5M (Linoleate), 6.6×10-5 M (Oleate),
  • 3.7×10-5M (Linolenate), 1.5×10-4 M (Palmitate),1.2×10-4M
  • (Myristate), 2.3×10-5 M (Stearate)
  • Inhibitors Hg2+, Ag+, ionic detergents
  • Optimum pH 7.0 - 9.0
  • Optimum temperature 40 °C
  • pH Stability pH 5.0 - 9.0 (25 °C, 24hr)
  • Thermal stability below 55 °C (pH 7.5, 10min)
  • EC # 3.1.1.13

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