Sorachim
Reference: COO-331

Cholesterol oxidase from Microorganism

Cholesterol oxidase from Microorganism Cholesterol + O2 ----> Choleste-4-en-3-one + H2O2 This enzyme is used for enzymatic determination cholesterol in serum when coupled with cholesterol esterase in clinical analysis.
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DESCRIPTION
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  • Product name: Cholesterol: oxygen oxidoreductase
  • Appearance: Yellowish lyophilized powder
  • Activity: Grade III 12 U/mg or more
  • Contaminants: Catalase: < 0.1 %
  • Cholesterol esterase: < 0.01 %
  • Stability: Stable at - 20 °C for at least one year
  • Stabilizers: Bovine serum albumin, amino acids
  • Molecular weight: Approx. 55 000 (by gel-filtration)
  • Isoelectric point: 4.6 ± 0.1 and 4.9 ± 0.1 and 5.2 ± 0.1
  • Michaelis constants: 3 x 10-5M (Cholesterol)
  • Inhibitors: Ionic detergents, Hg2+
  • Optimum pH: 7.0 - 8.0
  • Optimum temperature: 60 °C
  • pH Stability: pH 5.0 - 10.0 (25°C, 20hr)
  • Thermal stability: Below 60°C (pH 7.0, 15 min)
  • EC # 1.1.3.6

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