Reference: CHO-301

Choline oxidase

Choline oxidase from Alcaligenes sp. Choline + H20 + 2O2 ----> Betaine + 2H2O2 This enzyme is used for enzymatic determination of phospholipids when coupled with phospholipase D and for choline esterase-activity in clinical analysis.
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DESCRIPTION
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  • Appearance Yellowish amorphous powder lyophilized
  • Activity Grade Ⅲ10U/mg-solid or more (containing approx. 20% of
    stabilizers)
  • Contaminant Catalase ≤1.0×10-2%
  • Stabilizers EDTA, BSA ,amino acids, (glycinesodium glutamate etc.)
  • Stability Stable at - 20℃ for at least 6 months
  • Molecular weight approx. 95,000
  • Isoelectric point 4.1±0.1
  • Michaelis constants 2.84×10-3 M (Choline), 5.33×10-3M(Betaine aldehyde)
  • Structure One mol of FAD is covalently bound to mol of the enzyme)
  • Inhibitors p-Chloromercuribenzoate, Cu-2+ Co2+, Hg2+, Ag+
  • Optimum pH 8.0-8.5
  • Optimum temperature 40-45℃
  • pH Stability pH 7.0-9.0 (30℃, 2 hr)
  • Thermal stability below 37℃ (pH 7.5, 10min)
  • EC # 1.1.3.17

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