Sorachim
Reference: CRH-221

Creatinase from Microorganism

Creatinase from Actinobacillus sp. Creatine + H20 Sarcosine + Urea This enzyme is used for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase (CNH-211, CNH-311) sarcosine dehydrogenase or sarcosine oxidase (SAO-341) and formaldehyde dehydrogenase (FRD-201) in clinical analysis.
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DESCRIPTION
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  • Appearance White amorphous powder lyophilized
  • Activity Grade II 6.0U/mg-solid or more (containing approx.
    50% of stabilizers)
  • Contaminant NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0%
  • Stabilizers Sugars, EDTA
  • Stability Stable at 0-5℃ in a desiccators
  • Molecular weight approx. 100,000
  • Isoelectric point 4.6±0.1
  • Michaelis constant 1.9×10-2M (Creatine)
  • Structure 2 subunits per mol of enzyme
  • Inhibitors Cu2+, Hg2+, Ag+
  • Optimum pH 8.0
  • Optimum temperature 40℃
  • pH Stability pH 5.5-9.0 (25℃,16hr)
  • Thermal stability below 50℃ (pH 7.5,30min)
  • EC # 3.5.3.3

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