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Creatininase
Reference: CNH-211

Creatininase from Microorganism

Creatininase from microorganism Creatinine + H20 ----> Creatine This enzyme is used for enzymatic determination of creatinine when coupled with creatine amidinohydrolase (CRH-211, CRH-221), sarcosine dehydrogenase or sarcosine oxidase (SAO-341) in clinical analysis.
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DESCRIPTION
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  • Appearance: White amorphous powder lyophilized
  • Activity: Grade II 450 U/mg-solid or more
  • Contaminant: NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0%
  • Stabilizers: Sucrose, BSA
  • Stability: Stable at －20℃ for at least 12 months
  • Molecular weight: approx. 175,000
  • Isoelectric point: 4.7
  • Michaelis constants: 3.2×10-2 M (Creatinine), 5.7×10-2 M (Creatine)
  • Structure: 8 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
  • Inhibitors: Ag+, Hg2+ N-bromosuccinimide, EDTA
  • Optimum pH: 6.5－7.5
  • Optimum temperature: 70℃
  • pH Stability: pH 7.5－9.0 (5℃, 16hr)
  • Thermal stability: below 70℃ (pH 7.5, 30min)
  • EC # 3.5.2.10
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