Sorachim
Reference: CNH-211

Creatininase from Pseudomonas

Creatininase from Pseudomonas sp. Creatinine + H20 ----> Creatine This enzyme is used for enzymatic determination of creatinine when coupled with creatine amidinohydrolase (CRH-211, CRH-221), sarcosine dehydrogenase or sarcosine oxidase (SAO-341) and formaldehyde dehydrogenase (FRD-201) in clinical analysis.
Available quantities:
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
DESCRIPTION
Download
  • Appearance White amorphous powder lyophilized
  • Activity Grade III 150 U/mg-solid or more
  • Contaminant NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0%
  • Stabilizers Sucrose, BSA
  • Stability Stable at -20℃ for at least 6 months
  • Molecular weight approx. 175,000
  • Isoelectric point 4.7
  • Michaelis constants 3.2×10-2 M (Creatinine), 5.7×10-2 M (Creatine)
  • Structure 8 subunits per mol of enzyme (One mol of zinc isbound to each subunit)
  • Inhibitors Ag+, Hg2+ N-bromosuccinimide, EDTA
  • Optimum pH 6.5-7.5
  • Optimum temperature 70℃
  • pH Stability pH 7.5-9.0 (5℃, 16hr)
  • Thermal stability below 70℃ (pH 7.5, 30min)
  • EC # 3.5.2.10

We will solve any doubts you may have

Powered by VOLCÀNIC INTERNET
envelopephone-handset