Reference: CNI-311

Creatinine deiminase

Creatinine deiminase from Microorganism Creatinine + H₂0 ----> N-Metylidantoin + NH₄⁺ This enzyme is used for enzymatic determination of creatinine when coupled with glutamate dehydrogenase (GTD-211, GTD-409) in clinical analysis

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10ku

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DESCRIPTION

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Appearance: White amorphous powder lyophilized
Activity: Grade Ⅲ 10U/mg-solid or more (containing approx. 30%
of stabilizer)
Contaminant: Creatinine amidohydrolase ≤ 1.0×10^-2 %
Creatine amidinohydrolase ≤ 1.0×10^-2%
Urease ≤ 1.0×10^-2%
NADH oxidase ≤ 1.0×10^-2%
NH4+ ≤ 1.0×10^-2μg/u
Stabilizers: Mannitol
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 260,000
Isoelectric point: 4.4
Michaelis constant: 3.5×10^-3M (Creatinine)
Structure: 6 subunits per mol of enzyme
Inhibitors: Ag^＋,Hg^2＋, o-phenanthroline,monoiodoacetate
Optimum pH: 8.5－9.5
Optimum temperature: 65－75℃
pH Stability: pH 7.0－11.0 (30℃, 20hr)
Thermal stability: below 65℃ (pH 7.5, 1hr)
EC # 3.5.4.21

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Creatinine deiminase

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