Sorachim
Reference: CNI-311

Creatinine deiminase

Creatinine deiminase from Microorganism Creatinine + H20 ----> N-Metylidantoin + NH4+ This enzyme is used for enzymatic determination of creatinine when coupled with glutamate dehydrogenase (GTD-211, GTD-209, GTD-309) in clinical analysis
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DESCRIPTION
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  • Appearance White amorphous powder lyophilized
  • Activity GradeⅢ 10U/mg-solid or more (containing approx. 30%
    of stabilizer)
  • Contaminant Creatinine amidohydrolase ≤ 1.0×10-2 %
  • Creatine amidinohydrolase ≤ 1.0×10-2%
  • Urease ≤ 1.0×10-2%
  • NADH oxidase ≤ 1.0×10-2%
  • NH4+ ≤ 1.0×10-2μg/u
  • Stabilizers Mannitol
  • Stability Stable at -20℃ for at least 6 months
  • Molecular weight approx. 260,000
  • Isoelectric point 4.4
  • Michaelis constant 3.5×10-3M (Creatinine)
  • Structure 6 subunits per mol of enzyme
  • Inhibitors Ag+,Hg2+, o-phenanthroline,monoiodoacetate
  • Optimum pH 8.5-9.5
  • Optimum temperature 65-75℃
  • pH Stability pH 7.0-11.0 (30℃, 20hr)
  • Thermal stability below 65℃ (pH 7.5, 1hr)
  • EC # 3.5.4.21

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