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D-3-Hydroxybutyrate dehydrogenase
Reference: HBD-311

D-3-Hydroxybutyrate dehydrogenase

D-3-Hydroxybutyrate dehydrogenase from Microorganism D-3-hydroxybutyrate + NAD + ----> Acetoacetate + NADH + H+ D-3-hydroxybutyrate + NAD + <---- Acetoacetate + NADH + H+ This enzyme is used for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
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DESCRIPTION
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  • Product name: (R)-3-hydroxibutanoate: NAD+ oxidoreductase
  • Appearance: white amorphous powder lyophilized
  • Activity: 450 U/mg-solid or more
  • Contaminants: NADH oxidase ≤ 2.0×10－3%
    Malate dehydrogenase ≤ 2.0×10－3%
    Lactate dehydrogenase ≤ 2.0×10－3%
  • Stability: Stable at －20℃ for at least 12 months
  • Molecular weight: approx. 28,000 (SDS-PAGE)
  • Michaelis constants: 1.4 mM (37℃, pH 8.3), D-3-Hydroxybutyrate
    0.15 mM (37℃, pH 8.3), NAD+
  • Optimum pH: 8.0 - 8.5
  • Optimum temperature: Above 50 ℃
  • pH Stability: pH 5.0 - 10.5 (25 ℃, 20 h)
  • Thermal stability: below 40 ℃ (pH 6.5, 15 min)
  • EC # 1.1.1.30
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