Sorachim
Reference: HBD-301

D-3-Hydroxybutyrate dehydrogenase

D-3-Hydroxybutyrate dehydrogenase from Pseudomonas sp. D-3-hydroxybutyrate + NAD + ----> Acetoacetate + NADH + H+ D-3-hydroxybutyrate + NAD + <---- Acetoacetate + NADH + H+ This enzyme is used for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
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DESCRIPTION
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  • Product name (R)-3-hydroxibutanoate: NAD+ oxidoreductase
  • Appearance white amorphous powder lyophilized
  • Activity Grade III 100 U/mg-solid or more
  • Contaminants NADPH oxidase ≤ 2.0×10-3 %
  • Malate dehydrogenase ≤ 2.0×10-3%
  • Lactate dehydrogenase ≤ 2.0×10-3 %
  • Stabilizers Sucrose, manitol, BSA
  • Stability Stable at -20°C for at least one year
  • Molecular weight approx. 130,000 (by gel filtration)
  • Isoelectric point 5.6 ± 0.1
  • Michaelis constants 4.2×10-4M (25°C, pH8.3), 7.0×10-4M (37°C, pH8.3) (D-3-Hydroxybutyrate)
  • 4.9×10-5M (25°C, pH8.3), 7.2×10-5M (37°C, pH8.3) (NAD+)
  • 8.1×10-5M (25°C, pH7.1), 2.4×10-4M (37°C, pH7.1) (Acetoacetate)
  • 8.4×10-6M (25°C, pH7.1), 1.5×10-5M (37°C, pH7.1) (NADH)
  • Inhibitors PCMB, MIA, IAA, Ag+, Hg2+, SDS, DAC
  • Optimum pH 8.3
  • Optimum temperature 55°C
  • pH Stability pH 5.0-8.5 (25°C, 20hr)
  • Thermal stability below 40°C (pH 6.5, 15min)
  • EC # 1.1.1.30

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