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Reference: LCD-221

D-Lactate dehydrogenase

D-Lactate dehydrogenase from Microorganism D-Lactate + NAD+ ---> Pyruvate + NADH + H+ D-Lactate + NAD+ <--- Pyruvate + NADH + H+ This enzyme is used for enzymatic determination of numerous metabolites, e.g.ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g.GPT, PK and CPK when coupled with the related enzymes.
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DESCRIPTION
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  • Product name: (R)-Lactate: NAD+ oxidoreductase
  • Appearance: White amorphous powder lyophilized
  • Activity: Grade II 400U/mg-protein or more
  • Contaminants: Malate dehydrogenase ≤ 1.0×10-2%
  • Myokinase ≤ 1.0×10-2%
  • Pyruvate kinase ≤ 1.0×10-3%
  • NADH oxidase ≤ 1.0×10-3%
  • GOT ≤ 5.0×10-3%
  • GPT ≤ 5.0×10-3%
  • Stability: Store at -20°C for at least 12 months
  • Molecular weight: approx. 140,000
  • Isoelectric point: 4.0
  • Michaelis constant: 1.6 ×10-4 M (pyruvate, pH 7.0)
  • Inhibitors: Ag2+, Hg2+, SH-reagents
  • Optimum pH: 6.0-7.0
  • Optimum temperature: 35-40°C
  • pH Stability: pH 5.0 - 9.0 (25°C, 48hr)
  • Thermal stability: below 45°C (pH 7.0, 15min)
  • EC # 1.1.1.28
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