Reference: DAD-311

Diaphorase

Diaphorase from Clostridium NAD(P)H + H⁺ + Acceptor (ox) ----> NAD(P)⁺ + Acceptor (red) This enzyme is used for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.

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DESCRIPTION

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Name: NAD(P)H (acceptor) oxydoreductase
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 500 U/mg-solid or more
Contaminants: Myokinase: ≤ 5.0×10^-1 %, NADH oxidase: ≤ 1.0×10^{-1 %}
Stability: Stable at -20 °C for at least 12 months
Molecular: weight 48,000
Michaelis constants: 2.2×10^-4 M (NADH), 2.9×10^-2 M (NADPH)
Inhibitors: Fe³⁺, Mn²⁺, Cu²⁺, Pb²⁺
Isoelectric point: 5.0
Optimum: pH 8.0
Optimum temperature: 60°C
pH Stability: 5.0 – 10.0
Thermal stability: below 70°C
Substrate specificity: Either NADH or NADPH can be used as a reductant.
EC # 1.6.99.-

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Diaphorase

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