Glucose-6- phosphate dehydrogenase

This enzyme is also used for enzymatic determination of glucose when coupled with hexokinase (HXK-301, HXK-311)

• Name: D-Glucose-6-phosphate:NADP⁺ 1-oxydoreductase
• Appearance: white amorphous powder lyophilized
• Activity: Grade III, 200U/mg-solid or more
• Contaminants: Creatine phosphokinase ≤ 1×10^-3%
  Phosphoglucomutase ≤ 1×10^-3%
  6-Phosphogluconate dehydrogenase ≤ 5×10^-3%
  Phosphoglucose isomerase ≤ 1×10^-2%
  Glutathione reductase ≤ 1×10^-3%
  Hexokinase ≤ 1×10^-2%, Myokinase ≤ ×10^-2%
  NADH oxidase ≤ 1×10^-2%, NADPH oxidase ≤ 1×10^-2%
• Stability: Stable at -20°C for at least one year
• Molecular weight: 140,000 (Gel filtration)
• Michaelis constants: NAD^＋ linked :2.4×10^-4 M (NAD^＋) 2.4×10-4M (G-6-P)
  NADP^＋ linked :7.4×10^-6M (NADP^＋) 1.7×10^-5M (G-6-P)
• Inhibitors: Metal ions, Iodoacetamimide, SDS etc.
• Optimum pH: 7.8
• Optimum temperature: 50°C-55°C
• pH Stability: pH 5-11 (25°C, 22hr)
• Thermal stability: below 50°C (pH 7.8, 30min)
• EC # 1.1.1.49