Sorachim
Reference: G6D-321

Glucose-6- phosphate dehydrogenase

Glucose-6- phosphate dehydrogenase from Microorganism D-glucose-6-phosphate + NADP+ ----> D-glucono-δ-Lactone-6-phosphate + NADPH + H+ D-glucose-6-phosphate + NADP+ <---- D-glucono-δ-Lactone-6-phosphate + NADPH + H+ This enzyme is used for enzymatic determation of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase...
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DESCRIPTION
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This enzyme is also used for enzymatic determination of glucose when coupled with hexokinase (HXK-301, HXK-311)

  • Name D-Glucose-6-phosphate:NAD(P)+ 1-oxydoreductase
  • Appearance white amorphous powder lyophilized
  • Activity Grade III 200U/mg-solid or more
  • Contaminants Creatine phosphokinase ≤ 1×10-3%
  • Phosphoglucomutase ≤ 1×10-3%
  • 6-Phosphogluconate dehydrogenase ≤ 5×10-3%
  • Phosphoglucose isomerase ≤ 1×10-2% Glutathione reductase ≤ 1×10-3%
  • Hexokinase ≤ 1×10-2% Myokinase ≤ ×10-2%
  • NADH oxidase ≤ 1×10-2% NADPH oxidase ≤ 1×10-2%
  • Stability Stable at -20°C for at least one year
  • Molecular weight 140,000
  • Michaelis constants NAD+ linked :2.4×10-4 M (NAD+) 2.4×10-4M (G-6-P)
  • NADP+ linked :7.4×10-6M (NADP+) 1.7×10-5M (G-6-P)
  • Inhibitors Metal ions, Iodoacetamimide, SDS etc.
  • Optimum pH 7.8
  • Optimum temperature 50°C-55°C
  • pH Stability pH 5-11 (25°C 22hr)
  • Thermal stability below 50°C (pH 7.8 30min)
  • EC # 1.1.1.49

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