Reference: G6D-311

Glucose-6- phosphate dehydrogenase

Glucose-6- phosphate dehydrogenase from Leuconostoc mesenteroides D-glucose-6-phosphate + NADP+ ----> D-glucono-δ-Lactone-6-phosphate + NADPH + H+ D-glucose-6-phosphate + NADP+ <---- D-glucono-δ-Lactone-6-phosphate + NADPH + H+ This enzyme is used for enzymatic determation of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase...
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DESCRIPTION
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This enzyme is also used for enzymatic determination of glucose when coupled with hexokinase (HXK-301, HXK-311).

  • Name: D-Glucose-6-phosphate:NADP+ 1-oxydoreductase
  • Appearance: white amorphous powder lyophilized
  • Activity: Grade Ⅲ, 400U/mg-solid or more (NAD)
  • Contaminants: Creatine phosphokinase ≤ 1×10-3%, Phosphoglucomutase ≤ 1×10-3%
    6-Phosphogluconate dehydrogenase ≤ 5×10-3%
    Phosphoglucose isomerase ≤ 1×10-2%, Glutathione reductase ≤ 1×10-3%
    Hexokinase ≤ 1×10-2%, Myokinase ≤ ×10-2%
    NADH oxidase ≤ 1×10-2%, NADPH oxidase ≤ 1×10-2%
  • Stability: Stable at -20℃ for at least 12 months
  • Molecular weight: 104000(Gel filtration)
  • Isoelectric point: 4.6
  • Michaelis constants: NAD linked :1.06×10-4 M (NAD) 5.27×10-5M (G-6-P)
    NADP linked :5.69×10-6M (NADP) 8.1×10-5M (G-6-P)
  • Inhibitors: Acyl-CoA, ATP, metal ions
  • Optimum pH: 7.8
  • Optimum temperature: 50℃
  • pH Stability: pH 5.5-7.5 (30℃ 17hr)
  • Thermal stability: below 37℃ (pH 8.0 30min)
  • EC # 1.1.1.49

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