Sorachim
Reference: G6D-311

Glucose-6- phosphate dehydrogenase

Glucose-6- phosphate dehydrogenase from Leuconostoc mesenteroides D-glucose-6-phosphate + NADP+ ----> D-glucono-δ-Lactone-6-phosphate + NADPH + H+ D-glucose-6-phosphate + NADP+ <---- D-glucono-δ-Lactone-6-phosphate + NADPH + H+ This enzyme is used for enzymatic determation of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase...
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DESCRIPTION
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This enzyme is also used for enzymatic determination of glucose when coupled with hexokinase (HXK-301, HXK-311).

  • Name D-Glucose-6-phosphate:NAD(P)+ 1-oxydoreductase (EC 1.1.1.49)
  • Appearance white amorphous powder lyophilized
  • Activity GradeⅢ 400U/mg-solid or more (NAD+)
  • Contaminants Creatine phosphokinase ≤ 1×10-3%
  • Phosphoglucomutase ≤ 1×10-3%
  • 6-Phosphogluconate dehydrogenase ≤ 5×10-3%
  • Phosphoglucose isomerase ≤ 1×10-2% Glutathione reductase ≤ 1×10-3%
  • Hexokinase ≤ 1×10-2% Myokinase ≤ ×10-2%
  • NADH oxidase ≤ 1×10-2% NADPH oxidase ≤ 1×10-2%
  • Stability Stable at -20℃ for at least 6 months (powder form)
  • Stable at 5℃ for at least 6 months (liquid form)
  • Molecular weight 104000(two subunits of approx. 55000)
  • Isoelectric point 4.6 2)
  • Michaelis constants NAD+ linked :1.06×10-4 M (NAD+) 5.27×10-5M (G-6-P)
  • NADP+ linked :5.69×10-6M (NADP+) 8.1×10-5M (G-6-P)
  • Inhibitors Acyl-CoA, ATP, metal ions etc.
  • Optimum pH 7.8
  • Optimum temperature 50℃
  • pH Stability pH 5.5-7.5 (30℃ 17hr)
  • Thermal stability below 37℃ (pH 8.0 30min)
  • Substrate specificity Either NAD+ or NADP+ serves as coenzyme the reaction velocity with NAD+
  • being approximately 1.8 times greater than with NADP. D-Glucose-6-
  • phosphate is a preferential substrate for the enzyme although D-glucose
  • reacts slowly. Fructose-6-phosphate fructose- 6-diphoshate and ribose-5-
  • phosphate are not considered to be substrates.
  • EC # 1.1.1.49

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