Reference: GLD-311

Glucose-dehydrogenase (NADP(P)-dependent)

Glucose-dehydrogenase (NADP(P)-dependent) from Microorganism β-D-Glucose + NAD(P)+ ----> D-glucono-δ-Lactone + NAD(P)H + H+ This enzyme is used for enzymatic determination of D-Glucose
Available quantities:
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
DESCRIPTION
Download
  • Name Β-D-Glucose:NAD(P)+1-oxydoreductase
  • Appearance White amorphous powder lyophilized
  • Activity Grade III, 250U/mg-solid or more
  • Contaminants NADH oxidase ≤ 1.0×10-3 %
  • α-Glucosidase ≤ 1.0×10-3 %
  • Glucose-6-phosphate dehydrogenase ≤ 1.0×10-3 %
  • Stability Stable at - 20°C for at least one year
  • Molecular weight approx. 101,000 (Gel filtration)
  • Isoelectric point 4.5
  • Michaelis constants NAD+linked : 1.38×10-2 M (D-Glucose) 3.09×10-4 M (NAD+)
  • Inhibitors NADP+linked : 1.25×10-2M (D-Glucose) 4.07×10-5 M (NADP+)
  • Optimum pH Ag+, Hg2+, Monoiodoacetate
  • Optimum temperature 9.0
  • pH Stability 55°C
  • Thermal stability pH 6.0-7.5 (20°C, 16hr)
  • Substrate specificity 45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)
  • Specific for β-D-Glucose or 2-Deoxy-glucose (Either NAD+ or NADP+
  • serves as coenzyme.)
  • EC # 1.1.1.47

We will solve any doubts you may have

Powered by VOLCÀNIC INTERNET
envelopephone-handset