Reference: GLO-202A

Glucose-oxidase

Glucose oxidase from Aspergillus sp. β-D-glucose + O₂ + H₂O ----> D-glucono-δ-Lactone + H₂O₂ β-D-glucose + O₂ + H₂O <---- D-glucono-δ-Lactone + H₂O₂ This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis...

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DESCRIPTION

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This enzyme is also suitable for glucose strips
Name: β-D-Glucose:oxygen 1-oxydoreductase
Appearance: Freeze-dried yellow powder
Activity: 250 U/mg-solid or more - 1 unit oxidizes 1 μmole of β-D-glucose to D-gluconolactone and H₂O₂per
minute at pH 7.0 at 25°C
Contaminants: Catalase < 0.05 %
Amylase: < 0.05 %
Maltase: < 0.05 %
Saccharase: < 0.5 %
Stability: Stable at -20°C for at least one year
Molecular weight: approx. 153,000
Inhibitors: p-Chloromercuribenzoate, heavy metal ions (Cu²⁺, Hg²⁺, Ag⁺)
EC # 1.1.3.4

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Glucose-oxidase

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