Sorachim
Reference: GLO-201

Glucose-oxidase

β-D-glucose + O2 + H2O ----> D-glucono-δ-Lactone + H2O2 β-D-glucose + O2 + H2O <---- D-glucono-δ-Lactone + H2O2 This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis.
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DESCRIPTION
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  • Name β-D-Glucose:oxygen 1-oxydoreductase
  • Appearance Yellowish amorphous powder lyophilized
  • Activity Grade II 100U/mg-solid or more
  • (containing approx. 50% of stabilizers)
  • Contaminants Catalase ≤ 3.0%
  • Stabilizers Potassium gluconate, sodium glutamate
  • Stability Stable at -20℃ for at least one year
  • Molecular weight approx. 153,000
  • Michaelis constants 3.3×10-2 M (β-D-Glucose) 6.1×10-2M (2-Deoxyglucose)
  • Structure Glycoprotein with 2 moles of FAD
  • Inhibitors p-Chloromercuribenzoate, heavy metal ions (Cu2+, Hg2+, Ag+)
  • Optimum pH 4.5
  • Optimum temperature 40-50℃
  • pH Stability pH 4.5-6.0 (30℃, 20hr)
  • Thermal stability below 50℃ (pH 5.7, 1hr)
  • EC # 1.1.3.4

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