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Glutamate dehydrogenase
Reference: GTD-07C

Glutamate dehydrogenase

Glutamate dehydrogenase from Beef liver — GTD-07C (EC 1.4.1.3) L-Glutamate + H2O + NADP+ ----> 2-Oxoglutarate + NH3 + NADPH + H+ L-Glutamate + H2O + NADP+ <---- 2-Oxoglutarate + NH3 + NADPH + H+
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DESCRIPTION
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  • This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.
  • Product name: L-Glutamate:NAD(P)+ oxydoreductase (deaminating)
  • Appearance: White freeze-dried powder
  • Activity: > 10 U/mg-solid or more
  • Contaminants: NH4+ ≤ 1.0×10-3 μmol/U
    LDH ≤ 1.0×10-2 %
    MDH ≤ 1.0×10-2 %
  • Stability: Stable at -20°C for at least one year
  • Michaelis constants: 3.2×10-3 M (NH4+), 0.7×10-3M (α-Ketoglutarate),
    1.8×10-3M (L-Glutamate)
  • Inhibitors: Heavy metals, PCMB, IAA
  • EC # 1.4.1.3
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