Glutamic-oxaloacetic transaminase

Reference: GOT-30C

Glutamic-oxaloacetic transaminase

Glutamic-oxaloacetic transaminase from Porcine heart This enzyme is used in multi analyte clinical chemistry controls and calibrators.

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DESCRIPTION

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Name: L-Aspartate:2-oxoglutarate aminotransaminase. AST/ASAT.
Appearance: Yellow-green powder lyophilized
Activity: 50 u/mg-solid or more at 37°C
Contaminants: CK < 6×10^-3 %, ALT (GPT) < 7x10^-2 %, LDH < 6x10^-3 %
GTD < 0.2 %, MDH < 1.4x10^-2 %, Ammonia < 4x10^-2 %
Storage: Store at -20°C
Molecular weight: approx. 92 000
Unit definition: One unit will catalyze the transamination of 1 micromole of LAspartate and alpha-
ketoglutarate, forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8. Measured at 340nm as 1
equimolar amount of NAD produced by a coupled reaction.
Expiration: Stable at least 1 year at –20°C
EC # 2.6.1.1

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