Sorachim
Reference: GOT-30C

Glutamic-oxaloacetic transaminase

Glutamic-oxaloacetic transaminase from Porcine heart This enzyme is used in multi analyte clinical chemistry controls and calibrators.
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DESCRIPTION
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  • Name: L-Aspartate:2-oxoglutarate aminotransaminase. AST/ASAT.
  • Appearance: Yellow-green powder lyophilized
  • Activity: 50 u/mg-solid or more at 37°C
  • Contaminants: CK < 6×10-3 %, ALT (GPT) < 7x10-2 %, LDH < 6x10-3 %
  • GTD < 0.2 %, MDH < 1.4x10-2 %, Ammonia < 4x10-2 %
  • Storage: Store at -20°C
  • Molecular weight: approx. 92 000
  • Unit definition: One unit will catalyze the transamination of 1 micromole of LAspartate and alpha-
  • ketoglutarate, forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8. Measured at 340nm as 1
  • equimolar amount of NAD produced by a coupled reaction.
  • Expiration: Stable at least 1 year at –20°C 
  • EC # 2.6.1.1

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