Sorachim
Reference: GPT-30C

Glutamic-pyruvate transaminase

Glutamic-pyruvate transaminase from Porcine heart This enzyme is used in multi analyte clinical chemistry controls and calibrators.
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DESCRIPTION
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  • Name: L-Alanine:2-oxoglutarate aminotransaminase. ALT/ALAT.
  • Appearance: Yellow-green powder lyophilized
  • Activity: 70 u/mg-solid or more at 37°C
  • Contaminants: CPK < 8×10-4 %, AST (GOT) < 0.1 %, LDH < 8x10-3 %
  • GTD < 10-2 %, Protease < 2x10-3 %, Ammonia < 10-3 %
  • Storage: Store at -20°C
  • Molecular weight: approx. 100 000
  • Unit definition : One unit will catalyze the transamination of 1 micromole of LAlanine and alpha-ketoglutarate,
  • forming L-glutamate and pyruvate per minute at 37°C and pH 7.8. Measured at 340nm as 1 equimolar amount
  • of NAD produced by a coupled reaction.
  • Expiration: Stable at least 1 year at –20°C
  • EC # 2.6.1.2

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