Sorachim
Reference: GYD-301

Glycerol dehydrogenase

Glycerol dehydrogenase from Cellulomonas sp. Glycerol + NAD+ ----> Dihydroxiacetone + NADH + H+ Glycerol + NAD+ <---- Dihydroxiacetone + NADH + H+ This enzyme is used for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.
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DESCRIPTION
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  • Product name Glycerol:NAD+ 2-oxydoreductase
  • Appearance White amorphous powder lyophilized
  • Activity Grade III 50 U/mg-solid or more (containing 50% of stabilizers)
  • Contaminants NADH oxidase ≤ 1.0×10-3 %
  • Stabilizers BSA
  • Stability Stable at -20°C for at least six month
  • Molecular weight approx. 390000
  • Isoelectric point 4.4±0.1
  • Michaelis constants 1.1×10-2M (Glycerol) 8.9×10-5M (NAD+)
  • Structure 10 subunits (42000) per mol of enzyme
  • Inhibitors p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions
  • (Co2+, Ni2+, Cu2+, Zn2+, Cd2+)
  • Optimum pH 10.0-10.5
  • Optimum temperature 50°C
  • pH Stability pH 7.5-10.5 (25°C, 20hr)
  • Thermal stability below 55°C (pH 7.5, 15min)
  • Substrate specificity This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also
  • oxidizes glycerol-α-monochlorohydrin, ethylene glycol and 2,3-butanediol (Table 1).
  • The oxidative reaction is stimulated by K+, NH4+ and Rb+.
  • EC # 1.1.1.6

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