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Glycerol kinase
Reference: GYK-311

Glycerol kinase

Glycerol kinase from Microorganism Glycerol + ATP ----> Glycerol-3-phosphate + ADPMg2+ Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2+
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DESCRIPTION
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  • Product name: ATP: Glycerol 3-phosphotransferase
  • Appearance: White amorphous powder lyophilized
  • Activity: Grade Ⅲ 30 U/mg-solid or more
  • Contaminants: Catalase : ≤ 1.0x10-1 %, NADH oxidase : ≤ 1.0x10-3 %
    Adenosine triphosphate : ≤ 1.0x10-3 %
  • Stability: Stable at -20°C for at least 12 months
  • Molecular weight: approx. 220,000 (by gel filtration)
  • Structure: 4 subunits of approx 58,000
  • Isoelectric poin:t 4.3
  • Michaelis constants: 9.4×10-5 M (Glycerol), 1.3×10-5 M (ATP), 2.1x10-3 M (dihydroxiacetone)
  • Inhibitors: p-Chloromercuribenzoate, Hg++, Ag+
  • Optimum pH: 10.0
  • Optimum temperature: 70℃
  • pH Stability: pH 5.5 - 10.0 (25°C, 20hr)
  • Thermal stability: below 65℃ (pH 7.5, 30min)
  • EC # 2.7.1.30
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