Sorachim
Reference: GYK-311

Glycerol kinase

Glycerol kinase from Microorganism Glycerol + ATP ----> Glycerol-3-phosphate + ADPMg2+ Glycerol + ATP <---- Glycerol-3-phosphate + ADPMg2+
Available quantities:
If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
DESCRIPTION
Download
  • This enzyme is used for enzymatic determination of glycerol and triglyceride
  • when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase
  • (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD
  • 209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis
  • If you need bigger quantities than indicated on the web site, please contact us for a personal offer.
  • Product name ATP: Glycerol 3-phosphotransferase
  • Appearance White amorphous powder lyophilized
  • Activity Grade Ⅲ 30 U/mg-solid or more (containing approx. 50% stabilizers)
  • Contaminants NADH oxidase ≤ 1.0×10-3%
  • Catalase ≤ 1.0×10-2%
  • Phosphatase (pH 6) ≤ 1.0×10-3 %
  • Stability Stable at -20℃
  • Molecular weight approx. 222,000 (by gel filtration)
  • Isoelectric point 4.3
  • Michaelis constants 9.4×10-5M (Glycerol), 1.3×10-5M (ATP), 2.1x10-3M (dihydroxiacetone)
  • Inhibitors p-Chloromercuribenzoate, heavy metal ions (Pb2+, Fe2+, Hg2+, Ag+)
  • Optimum pH 10
  • Optimum temperature 70℃
  • pH Stability pH 5.5-10.0 (25℃, 20hr)
  • Thermal stability below 65℃ (pH 7.5, 30min)
  • EC # 2.7.1.30

We will solve any doubts you may have

Powered by VOLCÀNIC INTERNET
envelopephone-handset