Reference: HXK-311

Hexokinase

Hexokinase from Microorganism D-hexose + ATP ----> D-Hexose-6-Phosphate + ADP+ Mg2+ D-hexose + ATP <---- D-Hexose-6-Phosphate + ADP+ Mg2+ This enzyme is used for enzymatic determination of glucose and adenosine- 5'-triphosphate (ATP) when coupled with glucose-6-phosphate ehydrogenase G-6-PDH (G6D-311, G6D-321).
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DESCRIPTION
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  • Product name ATP: D-Hexose 6-phosphotransferase
  • Appearance White amorphous powder lyophilized
  • Activity Grade Ⅲ 150 U/mg-solid or more
  • Contaminants Phosphoglucose isomerase ≤ 1.0×10-1%
  • 6-Phosphogluconate dehydrogenase ≤ 1.0×10-2%
  • Glucose-6-phosphate dehydrogenase ≤ 1.0×10-2%
  • Myokinase ≤ 1.0×10-2%
  • Glutathione reductase ≤ 5.0×10-1%
  • Stability Store at - 20℃
  • Molecular weight approx. 82,000 (by gel filtration)
  • Michaelis constants 2.3×10-4 M (D-Glucose)7.7×10-5 M (ATP)
  • Inhibitors Metal ions p-chloromercur, ibenzoateiodoacetamide, SDS, etc
  • Activators Mg2+
  • Optimum pH 8.0 –9.0
  • Optimum temperature 50℃
  • pH Stability pH 4.0-9.0 (25℃, 20hr)
  • Thermal stability below 45℃ (pH 7.0, 30min)
  • EC # 2.7.1.1

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