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L-α-glycerophosphate oxidase
Reference: G3O-321

L-α-glycerophosphate oxidase

L-α-glycerophosphate oxidase from Microorganism Glycerol-3-phosphate + O2 ----> 3-Dihydroxyacetone phosphate + H2O2 This enzyme is used for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.
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DESCRIPTION
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  • Product name: sn-Glycerol 3-phosphotransferase: oxygen 2– oxidoredcutase
  • Appearance: Yellowish amorphous powder lyophilized
  • Activity: Grade III 15 U/mg-solid or more
  • Contaminants: Lactate oxidase ≤ 2.0×10-4 %
    Adenosine trihosphatase ≤ 5.0×10-4 %
  • Stabilizers: FAD, amino acids
  • Storage: Store at -20 °C for at least 12 months
  • Molecular weight approx. 67,000 (by SDS-PAGE)
  • Isoelectric point: 4.6±0.1
  • Michaelis constants: 1.3×10-3 M
  • Inhibitors: Ionic detergents, SH reagents, Metal ions, etc.
  • Optimum pH: 6.5 - 7.0
  • Optimum temperature: 45 °C
  • pH Stability: pH 4.5 - 8.5 (25 °C, 20hr)
  • Thermal stability: below 45 °C (pH 6.5, 15min)
  • EC # 1.1.3.21
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