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Lactate oxidase
Reference: LCO-301

Lactate oxidase

Lactate oxidase from Microorganism L-Lactate + O2 ----> Pyruvate + H2O2 L-Lactate + O2 <---- Pyruvate + H2O2 This enzyme is used for enzymatic determination of L-lactate.
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DESCRIPTION
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  • Product name: (L)-Lactate: oxygen oxidoreductase
  • Appearance: Yellowish amorphous powder lyophilized
  • Activity: > 80U/mg-protein or more
  • Contaminants: Pyruvate oxidase ≤ 1.0×10-3%
    Cholesterol oxidase ≤ 1.0×10-3%
    Uricase ≤ 1.0×10-3%
    Glucose oxidase ≤ 1.0×10-3%
  • Stability: Stable at - 20 °C for at least 12 months
  • Molecular weight: approx. 160,000 (by gel filtration)
  • Isoelectric point: 4.3 ± 0.2
  • Michaelis constant: 1.0×10-3 M (L-Lactate)
  • Inhibitors: Fe3+, SDS
  • Optimum pH: 7.5
  • Optimum temperature: 35 - 40 °C
  • pH Stability: pH 4.0 - 9.8 (25°C, 16hr)
  • Thermal stability: below 50°C (pH 7.0, 10min)
  • EC # 1.13.12.4
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