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Lipoproteine lipase
Reference: LPL-311

Lipoproteine lipase

Lipoproteine lipase from Pseudomonas sp. Triglyceride + 3H2O ----> Glycerol +3 fatty acid This enzyme is used for enzymatic determination of triglyceride in serum when coupled with...
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DESCRIPTION
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  • This enzyme is used for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5～3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
  • Product name: Triacylglycero-protein acylhydrolase
  • Appearance: Light brown amorphous powder lyophilized
  • Activity: Grade III, 20U/mg-solid or more (containing approx. 80% of stabilizers)
  • Contaminants: NADH oxidase ≤ 1.0×10-3 %
    Phosphatase ≤ 1.0×10-3 %
    Catalase ≤ 2.0×10-2%
    Cholesterol oxidase ≤ 2.0×10-3 %
  • Stabilizers: Mg2+, Na-cholate, bovine serum albumin
  • Stability: Stable at －20°C for at least one year
  • Molecular weight: approx. 134,000
  • Isoelectric point: 5.95 ± 0.05
  • Inhibitors: Hg2+, Ag+, ionic detergents
  • Optimum pH: 7.0－9.0
  • Optimum temperature: 45－50°C
  • pH Stability: pH 7.0－9.0 (25°C, 20hr)
  • Thermal stability: below 55°C (pH 7.0, 10min)
  • EC # 3.1.1.34
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