Reference: MAD-211

malate dehydrogenase

Malate dehydrogenase from Microorganism L-Malate + NAD+ ----> Oxaloacetate + NADH + H+ L-Malate + NAD+ <---- Oxaloacetate + NADH + H+ This enzyme is used for enzymatic determination of L-malate and of glutamate oxaloacetate transaminase (GOT) in clinical analysis
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DESCRIPTION
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  • Product name (s)-malate: NAD+ oxydoreductase
  • Appearance White amorphous powder lyophilized
  • Activity Grade II 40U/mg-solid or more
  • Contaminants Glutamate oxaloacetate transaminase ≤ 1.0×10-3 %
  • Lactate dehydrogenase ≤ 1.0×10-3 %
  • NADH oxidase ≤ 1.0×10-3 %
  • Stability Stable at - 20°C for at least 12 months
  • Molecular weight Approx. 140,000
  • Isoelectric point pH 4.8 ± 0.1
  • Michaelis constants 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate), 8.1×10-6M
    (NADH)
  • Structure 4 subunits per mole of enzyme
  • Inhibitors Hg2+
  • Optimum pH 8.0
  • Optimum temperature 70°C
  • pH Stability pH 3.0 - 9.0 (25°C, 20hr)
  • Thermal stability Below 70°C (pH 7.5, 15min)
  • EC # 1.1.1.37

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