p-Hydroxybenzoate hydroxylase

Reference: HBH-311

p-Hydroxybenzoate hydroxylase

p-Hydroxybenzoate hydroxylase from Microorganism 4-hydroxybenzoate, NADPH:oxygen oxidoreductase (3-hydroxylating) EC # 1.14.13.2

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Product name: 4-hydroxybenzoate, NADPH:oxygen oxidoreductase (3-hydroxylating)
EC 1.14.13.2
Appearance: Yellowish amorphous powder lyophilized
Activity: Grade III, 20 U/mg-solid or more (containing approx. 40% stabilizers)
Contaminants: NADPH oxidase ≤ 1.0×10^-1%
Stabilizers: Sugars, FAD
Stability: Stable at －20℃ for at least 12 months
Molecular weight: 55,000～60,000
Michaelis constants: 2.0×10^-5 M (p-Hydroxybenzoate), 4.0×10^-5M (NADPH)
Structure: One mol of FAD per mol of enzyme
Inhibitors: Ag⁺, Hg²⁺, PCMB, SDS
Optimum pH: 7.7－7.9
Optimum temperature: 35℃
pH Stability: pH 5.0－7.5 (25℃, 72hr)
Thermal stability: below 40℃ (pH 6.0, 15min)

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