Reference: PNP-301

Purine-nucleoside phosphorylase

Purine-nucleoside : orthophosphate ribosyltransferase from Microorganism EC 2.4.2.1  
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DESCRIPTION
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  • Name Purine-nucleoside: orthophosphate ribosyltransferase
  • EC 2.4.2.1
  • Appearance White amorphous powder lyophilized
  • Activity Grade III, 15 U/mg-solid or more
  • Contaminants Catalase ≤ 20%
  • 5'-Nucleosidase ≤ 1.0×10-3 %
  • Adenosine deaminase ≤ 1.0×10-3 %
  • ATPase ≤ 1.0×10-2 %
  • Stabilizers K-Gluconate, mannitol, EDTA
  • Stability Stable at - 20℃ for at least 12 months
  • Molecular weight Approx. 120,000
  • Isoelectric point 4.1±0.1
  • Michaelis constants 6.4×10-5 M (Inosine), 3.2×10-4 M (Pi)
  • Inhibitors p-Chloromercuribenzoate, SDS, Hg2+, Ag+
  • Optimum pH 7.5-8.0
  • Optimum temperature 65℃
  • pH Stability pH 6.0-9.0 (30℃, 16hr)
  • Thermal stability Below 60℃ (pH 7.7, 30min)

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