Reference: SAO-341

Sarcosine oxidase

Sarcosine oxidase from Microorganism Sarcosine + H2O +O2 ----> Glycine + Formaldehyde + H2O2 Sarcosine + H2O +O2 <---- Glycine + Formaldehyde + H2O2 This enzyme is used for enzymatic determination of creatinine, creatine, and sarcosine when coupling with...
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DESCRIPTION
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This enzyme is used for enzymatic determination of creatinine, creatine, and sarcosine when coupling with creatinine amidohydrolase (CNH-211, CNH-311) and creatine amidinohydrolase (CRH-211, CRH-221).-341 is newer type of sarcosine oxidase, with improved stability in antimicrobial reagent.

  • Name: Sarcosine: oxygen oxidoreductase (demethylating)
  • Appearance: Yellowish amorphous powder lyophilized
  • Activity: Grade Ⅲ, 8U/mg-solid or more
  • Contaminants: Catalase ≤ 1%
  • Stabilizers: Potassium Gluconate
  • Stability: Stable at - 20℃ for at least one year
  • Molecular weight: Approx. 65,000 (by gel filtration)
  • Isoelectric point: 4.9±0.1
  • Michaelis constants: 2.8×10-3 M (Sarcosine)
  • Inhibitors: Cu2+, Ag+, Hg2+, p-chloromercuribenzoate, N-ethylmaleimide, SDS
  • Optimum pH: 7.0-8.5
  • Optimum temperature: 40-50℃
  • pH Stability: pH 6.5-9.0 (25℃, 24hr)
  • Thermal stability: below 55℃ (pH 7.5, 10min)
  • EC # 1.5.3.1

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