Urease

Reference: URH-10S

Urease

Urease from Jack Bean (NH₂)₂CO+ H₂O ---> 2NH₃ + CO₂ This enzyme is used for enzymatic determination of urea in clinical analysis.

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DESCRIPTION

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Name: Urea amidohydrolase
Appearance: white amorphous powder lyophilized
Activity: 220U/mg-solid or more (Nessler units)
Contaminants: NH4+ ≤ 2.0×10^-4 μmol/U
Stability: Stable at -20°C for at least 6 months
Molecular weight: approx. 480 000
Isoelectric point: 5.0—5.1
Michaelis constant: 1.05×10^-2 M (Urea)
Structure: 8 active sites with SH-groups per mole of the enzyme
Inhibitors: Heavy metal ions (Ag⁺ Hg²⁺ etc.)
Optimum pH: 6.0
Optimum temperature: 60°C
pH Stability: pH 5.5—8.5 (30°C, 17hr)
Thermal stability: below 50°C, (pH 8.0, 60min)
EC # 3.5.1.5

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