Reference: UAO-211

Uricase

Uricase from Bacillus sp. Urate + O2 + H2O ----> Allantoin + CO2 + H2O2 This enzyme is used for enzymatic determination of uric acid in clinical analysis.
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DESCRIPTION
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  • Name: Urate: oxygen oxidoreductase
  • Appearance: white amorphous powder lyophilized
  • Activity: Grade II, 1.5U/mg-solid or more
  • Contaminants: Catalase ≤ 1.0%
  • Stabilizers: Borate, EDTA, nonionic detergents
  • Stability: Stable at - 20 °C for at least 12 months
  • Molecular weight: approx. 150,000
  • Structure: 4 subunits per molecule
  • Isoelectric point: 4.7
  • Michaelis constant: 1.36×10-5 M (Uric acid)
  • Inhibitors: Ag+, Hg2+
  • Optimum pH: 8.5
  • Optimum temperature: 45 °C
  • pH Stability: pH 6.0-9.5 (25 °C, 20hr)
  • Thermal stability: below 60 °C (pH 8.0, 10min)
  • EC # 1.7.3.3

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