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Xantine oxidase
Reference: XTO-212

Xantine oxidase

Xantine oxidase from Microorganism hypoxantine + O2 + H2O ----> Xantine + H2O2 Xantine + O2 + H2O ----> Uric Acid + H2O2 This enzyme is used for enzymatic determination of inorganic phosphorus, 5'nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-301) and uricase (UAO-201, UAO-211).
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DESCRIPTION
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  • Name: Xantine: oxygen oxidoreductase
  • Appearance: Reddish brown amorphous powder, lyophilized
  • Activity: Grade II, 10U/mg-solid or more
  • Contaminants: Catalase ≤ 5.0 %, Adenosine deaminase ≤ 1.0×10-3 %, Uricase ≤ 1.0×10-3 %, Phosphatase ≤ 1.0×10-3 %, Purine-nucleoside phosphorylase ≤ 5.0×10-3 %
  • Stabilizers: Sodium glutamate, BSA
  • Stabilit:y Stable at - 20°C for at least 12 months
  • Molecular weight: approx. 160,000
  • Isoelectric point: 4.0 ± 0.1
  • Michaelis constants: 4.5×10-5M (Xanthine), 7.6×10-5M (Hypoxanthine)
  • Inhibitors: Reducing agents, Hg2+, Ag+,MIA
  • Optimum pH: 7.5 - 8.0
  • Optimum temperature: 65°C
  • pH Stability: pH 6.5 - 9.0 (25°C, 15hr)
  • Thermal stability: below 55°C (pH 8.0, 30min)

EC # 1.1.3.22

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