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Glucose-dehydrogenase (PQQ-dependent)
Reference: GLD-321

Glucose-dehydrogenase (PQQ-dependent)

Glucose-dehydrogenase (PQQ-dependent) from Microorganism D-Glucose + Acceptor (ox) ----> D-glucono-1,5-Lactone + Acceptor (red) This enzyme is used for enzymatic determination of D-Glucose
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DESCRIPTION
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  • Name: D-Glucose:(pyrroloqinoline-quinone) 1-oxydoreductase
  • Appearance: Purple amorphous powder lyophilized
  • Activity: Grade III, 500U/mg-solid or more
  • Contaminants: Glucose dehydrogenase (NAD-dependent) ≤ 1.0×10-3 %, Hexokinase ≤ 1.0×10-3 %
  • Stability: Store at - 20°C for at least 12 months
  • Stabilizers: Ca++, BSA
  • Molecular weight: approx. 100,000 (by gel filtration）
  • Michaelis constant: 4.8 mM (D-Glucose)
  • Inhibitors: Cu2+ ,Pb2+, Ag+
  • Optimum pH: 7.0
  • Optimum temperature: 37°C
  • pH Stability: pH 3.5 - 8.5 (25°C, 16hr)
  • Temperature stability: below 50°C (pH 7.5, 30min)
  • EC # 1.1.5.2
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